p53 anticorps (AA 20-25)

N° du produit ABIN198282

Cet anticorps anti-p53 est un anticorps Souris Monoclonal détectant p53 dans WB, IHC, FACS, IP, IHC (p) et IHC (fro). Adapté pour Humain et Boeuf (Vache).

Aperçu rapide pour p53 anticorps (AA 20-25) (ABIN198282)

Antigène: p53 (TP53) (Tumor Protein P53 (TP53))

Reactivité: Humain, Boeuf (Vache)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp p53 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: DO-7

Détail du produit anti-p53 anticorps

Épitope: AA 20-25

Specificité: Recognizes the human 53kD p53 tumor suppressor protein, also known as Cellular tumor antigen p53 or Antigen NY-CO-13, encoded by the TP53 gene. p53 is a 393 amino acid protein with an N-terminal transactivation domain, followed by a proline-rich region and a DNA binding domain in the central core region. The C-terminal region contains a tetramerization domain and a terminal regulatory domain. p53 is intimately involved in a number of signaling pathways controlling cell division, cycling and apoptosis and is thus a potent cancer suppressor. In normal cells the level of p53 expression is low but can be induced by DNA damage or other stress signals. Activation of p53 leads to growth arrest through its interaction with p21, GADD45 and, DNA repair and potentially apoptosis through interaction with, and. p53 is critically regulated by Mdm2 which can trigger p53 degradation by a ubiquitin dependent systemrecognizes an epitope at the N-terminal end of p53 between amino acids 20-25, binding to both wild type and mutant forms. Clone DO-7 is not expected to recognize the multiple lacking the N-terminal region.

Aucune reactivité croisée: Souris, Rat (Rattus)

Purification: Purified IgG

Immunogène: Recombinant human p53 ( AA 20-25 ).

Type of Immunogen: Recombinant protein

Isotype: IgG2b

Information d'application

Indications d'application: Approved: Flo (1:50 - 1:100), IHC, IHC-Fr, IHC-P (1:1000), IP, WB (1:1000)

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 whole blood in 100 μL. Membrane permeabilization is required for this application. Immunohistochemistry: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer  pH 6.0 is recommended for this purpose. Histology Positive Control Tissue: Colon or breast carcinoma.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Détails sur p53

Antigène: p53 (TP53) (Tumor Protein P53 (TP53))

Autre désignation: TP53 / p53

Sujet: Name/Gene ID: TP53
Family: P53

Synonyms: TP53, BCC7, Cellular tumor antigen p53, p53, TRP53, p53 tumor suppressor, Phosphoprotein p53, Antigen NY-CO-13, Tumor protein p53, Tumor suppressor p53, LFS1

ID gène: 7157

Pathways: Signalisation p53, Signalisation MAPK, Signalisation PI3K-Akt, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, L'effet Warburg